Enrichment performance and salt tolerance of polyhydroxyalkanoates (PHAs) producing mixed cultures under different saline environments.

The key to the resource recycling of saline wastes in form of polyhydroxyalkanoates (PHA) is to enrich mixed cultures with salt tolerance and PHA synthesis ability. However, the comparison of saline sludge from different sources and the salt tolerance mechanisms of salt-tolerant PHA producers need to be clarified. In this study, three kinds of activated sludge from different salinity environments were selected as the inoculum to enrich salt-tolerant PHA producers under aerobic dynamic feeding (ADF) mode with butyric acid dominated mixed volatile fatty acid as the substrate. The maximum PHA content (PHAm) reached 0.62 ± 0.01, 0.62 ± 0.02, and 0.55 ± 0.03 g PHA/g VSS at salinity of 0.5%, 0.8%, and 1.8%, respectively. Microbial community analysis indicated that Thauera, Paracoccus, and Prosthecobacter were dominant salt-tolerant PHA producers at low salinity, Thauera, NS9_marine, and SM1A02 were dominant salt-tolerant PHA producers at high salinity. High salinity and ADF mode had synergistic effects on selection and enrichment of salt-tolerant PHA producers. Combined correlation network with redundancy analysis indicated that trehalose synthesis genes and betaine related genes had positive correlation with PHAm, while extracellular polymeric substances (EPS) content had negative correlation with PHAm. The compatible solutes accumulation and EPS secretion were the main salt tolerance mechanisms of the PHA producers. Therefore, adding compatible solutes is an effective strategy to improve PHA synthesis in saline environment.

Effects of Fe/Fe-Mn oxides loaded biochar on anaerobic degradation of typical phenolic compounds in coal gasification wastewater: Performance and mechanism.

In this study, two kinds of magnetic biochar (BC) were synthesized by loading Fe (FeBC) and Fe-Mn oxides (FMBC) and their effects on anaerobic phenolics degradation were investigated. Compared with BC/FMBC, FeBC addition achieved the superior phenolics biodegradation even for 3,5-xylenol. Compared with control, FeBC addition enhanced CH4 production by 100.1 % with the lag time shortened from 9.5 days to 6.6 days while it increased to 11.2 days with FMBC addition. FeBC addition activated adsorption-biodegradation and Fe (III) reduction with the improved electron transfer activity, adenosine triphosphate and cytochrome C concentrations. Abundant phenol degrading bacteria, electroactive bacteria, syntrophic partners could be enriched by FeBC addition, contributing to the enhanced benzoyl-CoA and methanogenesis pathways. However, this enhancement was inhibited by FMBC addition owing to the accumulation of reactive oxygen species. This study provided novel insights into the application of magnetic BC to enhanced anaerobic treatment of phenolic wastewater.

Effects of oxytetracycline on variation in intracellular and extracellular antibiotic resistance genes during swine manure composting.

This research aimed to investigate the alterations in extracellular (eARGs) and intracellular (iARGs) antibiotic resistance genes in response to oxytetracycline (OTC), and unravel the dissemination mechanism of ARGs during composting. The findings revealed both low (L-OTC) and high contents (H-OTC) of OTC significantly enhanced absolute abundance (AA) of iARGs (p < 0.05), compared to CK (no OTC). Composting proved to be a proficient strategy for removing eARGs, while AA of eARGs was significantly enhanced in H-OTC (p < 0.05). OTC resulted in an increase in AA of mobile genetic elements (MGEs), ATP levels, antioxidant and DNA repair enzymes in bacteria in compost product. Structural equation model further demonstrated that OTC promoted bacterial DNA repair and antioxidant enzyme activities, altered bacterial community and enhanced MGEs abundance, thereby facilitating iARGs dissemination. This study highlights OTC can increase eARGs and iARGs abundance, underscoring the need for appropriate countermeasures to mitigate potential hazards.

Ecological succession and community assembly of mixed microbial culture polyhydroxyalkanoate production systems: Drivers of polyhydroxyalkanoate synthesis and Bdellovibrio predation.

The production of polyhydroxyalkanoate (PHA) by mixed microbial culture (MMC) can reduce the pollution of plastics. Ecophysiological study of the microbial community assembly and succession is helpful for comprehensive understanding the MMC PHA production process. The operation mode of sequential aerobic dynamic discharge - aerobic dynamic feeding (ADD-ADF) was applied and the operation can be divided into acclimation phase and maturation phase. Deterministic process caused by selective pressure dominated the community assembly throughout the operation. In the acclimation phase, the physical selective pressure recovered the settling capacity of the system, and settling ability of the MMC was closely related to function of PHA synthesis. However, in the maturation phase, stochastic process caused sludge bulking, making the settling ability and PHA synthesis function of the MMC independent on each other. Stochastic process led to the succession of the dominant PHA-producing bacteria, for example, the predation of Paracoccus and Thauera by Bdellovibrio.

Enhanced anaerobic biodegradation of typical phenolic compounds in coal gasification wastewater (CGW) using biochar: Focusing on the hydrolysis-acidification process and microbial community succession.

The aim of this research is to investigate the effects of biochar (BC) on treatment performance (especially hydrolysis-acidification process) and microbial community shifts during anaerobic degradation of typical phenolic compounds in coal gasification wastewater. Compared to the control group, the removal of phenol, p-cresol and 3, 5-xylenol was gradually enhanced when increasing the BC addition within the test dosage (1-5 g/L). The biodegradation of phenol and p-cresol was significantly enhanced by BC addition while limited improvement for 3, 5-xylenol. The addition of BC significantly accelerated the hydrolysis-acidification process with the hydrolytic removal of phenol improved by 69.14%, the microbial activity was enhanced by 57.01%, and the key hydrolase bamA gene was enriched by 117.27%, respectively. Compared to 1-2 g/L dose, more protein-like and humic acid-like substances were secreted with 5 g/L BC, which probably contributed to higher extracellular electron transfer efficiency. In addition, phenol degrading bacteria (Syntrophorhabdus, Dysgonomonas, Holophaga, etc.) and electroactive microorganisms (Geobacter, Syntrophorhabdus, Methanospirillum, etc.) were enriched by BC addition. The functional genes related to carboxylation, benzoylation and ring cleavage processes of benzoyl-CoA pathway were potentially activated by BC.

Coupled pyrite and sulfur autotrophic denitrification for simultaneous removal of nitrogen and phosphorus from secondary effluent: feasibility, performance and mechanisms.

The discharge standards of nitrogen (N) and phosphorus (P) in wastewater treatment plants (WWTPs) have become increasingly strict to reduce water eutrophication. Further reducing N and P in effluent from municipal WWTPs need to be achieved effectively and eco-friendly. In this study, a carbon independent pyrite and sulfur autotrophic denitrification (PSAD) system using pyrite and sulfur as electron donor was developed and compared with pyrite autotrophic denitrification (PAD) and sulfur autotrophic denitrification (SAD) systems through batch and continuous flow biofilter experiments. Compare to PAD and SAD, PSAD was more effective in simultaneous removal in N and P. At hydraulic retention time (HRT) 3 h, average effluent concentrations of total nitrogen (TN) and total phosphate (TP) of 1.40 ± 0.03 and 0.19 ± 0.02 mg/L were achieved when treating real secondary effluent with 20.65 ± 0.24 mg/L TN and 1.00 ± 0.24 mg/L TP. The improvement in simultaneous removal of N and P was attributed to the coupling of PAD and SAD in enhancing the transformation of sulfur and iron and enlarging the reaction zone in the pyrite and sulfur autotrophic denitrification biofilter (PSADB) system. Therefore, more biomass was accumulated and the microbial denitrification functional stability, including electrons transfer and consumption was enhanced on the surface of pyrite and sulfur particles in the PSADB system. Moreover, autotrophic denitrifiers (Thiobacillus and Ferritrophicum), sulfate-reducing bacteria (Desulfocapsa) and iron reducing bacteria (Geothrix), acting as contributors to microbial nitrogen, sulfur and iron cycle, were specially enriched. In addition, the leaching of iron ions was promoted, which facilitated the removal of phosphate in the form of Fe3(PO4)2·8H2O and Fe3PO4. PSADB has proven to be an efficient technology for simultaneous removal of N and P, which could meet increasingly stringent discharge standards effectively and eco-friendly.

Simultaneous passivation of heavy metals and removal of antibiotic resistance genes by calcium peroxide addition during sewage sludge composting.

This research evaluated the effects of calcium peroxide (CP) at 0% (CK, w/w), 5% (T1, w/w), and 10% (T2, w/w), on heavy metals (HMs) mobility and prevalence of antibiotic resistance genes (ARGs) during sludge composting. T1 and T2 significantly reduced (p < 0.05) the mobility of Cu (29.34%, and 32.94%, respectively), Ni (24.07%, and 31.48%, respectively) and Zn (33.28%, and 54.11%, respectively) compared to CK after the composting. CP addition resulted in a decrease in mobile genetic elements (MGEs) and ARGs during composting. Together with structural equation model and random forest analysis depicted MGEs had a primary association with total ARGs variations during composting. Microbial analysis indicated CP downregulated the expression of the genes associated with two-component and type IV secretion system, thus reducing the prevalence of ARGs. This study demonstrates that application of CP is a feasible strategy to mitigate both ARGs and HMs hazards during composting.

Effect of calcium peroxide dosage on organic matter degradation, humification during sewage sludge composting and application as amendment for Cu (II)-polluted soils.

In this research, it was the first time to investigate the effect of two dosages (5% (T1) and 10% (T2), w/w) of calcium peroxide (CP) on organic matter degradation, humification during sewage sludge composting. Additionally, the complexation of Cu to humic substance (HS) derived from CP-compost compared to no CP addition-compost (CK) was also studied. Results showed that compared to CK, T1 and T2 significantly enhanced organic matter degradation and promoted the formation of HS. Two-dimensional correlation Fourier transform infrared spectroscopy (2D-FTIR-COS) and Parallel factor (PARAFAC) analysis revealed that the addition of CP accelerated the synthesis of HS with high aromatization degree and molecular weight than those in CK, owing to the oxidation of small molecules to form carboxyl. The stability constant (log KM) of Cu with CP-derived HS (log KM = 4.22-5.13) indicated a greater complexation ability than CK-derived HS (log KM = 4.05-4.45), due to the faster response of polysaccharides binding to Cu (II) and the higher humification degree of CP-derived HS. This study revealed the potential mechanisms of CP addition on the synthesis of HS and utilization of CP-compost product might provide an effective way to remedy Cu (II)-contaminated soils.

New insights into the transformation of effluent organic matter during Fe(II)-assisted advanced oxidation processes: Parallel factor analysis coupled with self-organizing maps.

The negative effects of effluent organic matter (EfOM) on receiving aquatic environments and advanced treatment facilities pose significant concerns. However, the effective removal of EfOM is challenging due to its chemically complex nature and its refractory characteristics. In this study, two Fe(II)-assisted oxidation processes including UV/Fe(II)/H2O2 and UV/Fe(II)/persulfate (UV/Fe(II)/PS) were investigated to promote EfOM reduction. Fe(II) was essential for promoting EfOM degradation. The mineralization rate of EfOM increased from 7 to 29% with 2 mM Fe(II) addition in the UV/H2O2 process and to 23% with 0.8 mM Fe(II) addition in the UV/PS process. A preliminary experiment was conducted to obtain the optimal molar ratio of oxidant to Fe(II) for practical applications based on different indicators. The form of Fe(III) prevalent at different pH values strongly affected Fe(II)/Fe(III) cycling, thus determining the progress of EfOM degradation. A machine learning approach consisting of parallel factor analysis coupled with self-organizing maps (PARAFAC-SOM) was employed with fluorescence spectra to visualize the degradation behavior of EfOM in the different reaction systems. Four components (i.e., two humic-like substances, one fulvic acid, and one tryptophan-like substance) were eventually identified, and their reductions reached more than 62% during the Fe(II)-assisted oxidation processes. The degradation orders for each component in the different oxidation processes were initially evaluated by SOM analysis with Fmax percentage data. The degradation behavior of EfOM in the UV/Fe(II)/H2O2 and UV/Fe(II)/PS systems exhibited different trends based on the best matching unit map and component planes. The humic-like component was more refractory than the other three components in both oxidation processes. The microbial humic-like and high-molecular-weight fulvic acid substances showed higher reactivity with SO·4- than with ·OH, while the tryptophan-like substance was more reactive in the UV/Fe(II)/H2O2 system than in the UV/Fe(II)/PS system. The outcomes of this study provide new insights into the degradation behavior of EfOM, promoting the development of advanced wastewater treatments.

Biochar induced inhibitory effects on intracellular and extracellular antibiotic resistance genes in anaerobic digestion of swine manure.

Distribution of intracellular (iARGs) and extracellular ARGs (eARGs) in manure anaerobic digestion (AD) process coupled with two types of biochar (BC and BP) were investigated. And the effects of biochar on the conjugation transfer of ARGs were explored by deciphering the interaction of biochar with bacterial stress responses, physiological metabolism and antibiotic resistances. Results showed that AD process could effectively remove all the detected eARGs with efficiency of 47.4-98.2%. The modified biochar (BP) with larger specific surface area (SSA) was propitious to decrease the absolute copy number of extracellular resistance genes. AD process could effectively remove iARGs by inhibiting the growth of host bacteria. The results of structural equation models (SEM) indicated that biochar put indirect influences on the fate of ARGs (λ = -0.23, P > 0.05). Analysis on oxidative stress levels, antioxidant capacity, DNA damage-induced response (SOS) response and energy generation process demonstrated that biochar induced the oxidative stress response of microorganisms and enhanced the antioxidant capacity of bacteria. The elevated antioxidant capacity negatively affected SOS response, amplified cell membrane damage and further weakened the energy generation process, resulted in the inhibition of horizontal transfer of ARGs.

Potassium ferrate combined with ultrafiltration for treating secondary effluent: Efficient removal of antibiotic resistance genes and membrane fouling alleviation.

Antibiotic resistance genes (ARGs) are considered as emerging environmental contaminants, which should be controlled by wastewater treatment plants to prevent their discharge into the environment. However, conventional treatment techniques generally fail to successfully reduce ARGs, and the release of cell-free ARGs was underestimated. In this study, potassium ferrate (Fe(VI)) pretreatment combined with ultrafiltration (UF) process was developed to remove both cell-associated and cell-free ARGs in real secondary effluent, compared to ferric chloride (Fe(III)) and poly-aluminum chloride (PACl) pretreatment processes. It was found that total ARGs especially cell-free ARGs were effectively removed by Fe(VI) oxidation. However, due to the poor settleability of the negatively charged particles formed by Fe(VI) in the secondary effluent, the removal of cell-associated ARGs was less compared to Fe(III) and PACl pretreatments. The combination of Fe(VI) and UF removed the most ARGs (3.26 - 5.01 logs) due to the efficient removal of cell-free ARGs by Fe(VI) (> 2.15 logs) and co-interception of both cell-associated ARGs and Fe(VI) formed particles of the UF. High-throughput sequencing revealed that Fe(VI) decreased the viability and relative abundances of the potential ARGs hosts. Fe(VI)-UF exhibited the best performance on humic-like fluorescent organic matters removal, as well as the least phytotoxicity in the effluent. Moreover, membrane fouling was remarkably alleviated by Fe(VI) pretreatment because (1) Fe(VI) removed macromolecules such as protein-like and polysaccharide-like substances which would block the membrane pores, (2) Fe(VI) improved the hydrophilicity of foulants and reduced the hydrophobic adsorption between foulants and membrane. In short, Fe(VI)-UF is a promising technology to efficiently remove ARGs (especially cell-free ARGs) and alleviate UF membrane fouling in wastewater reclamation.

Rapid recovery of mixed culture polyhydroxyalkanoate production system from EPS bulking using azithromycin.

The long-term stable operation of the mixed culture polyhydroxyalkanoate (PHA) enrichment stage is the guarantee for the continuous synthesis of PHA, however extracellular polymeric substances (EPS) sludge bulking occurred from time to time may cause the operation fail. In order to solve this problem, as a quencher of signal molecules and antibiotic, azithromycin (AZM) was used in the two systems with different modes to recover the sedimentation capacity of the sludge. The results showed that AZM addition resulted in the reduction of polysaccharide /protein (PS/PN) ratio in EPS and significant improvement of the sedimentation capacity of the sludge. Quorum quenching of AZM or aiiA gene maintained the sedimentation ability of the sludge in a relay mode. By adding AZM, the growth of Thauera and Flavobacterium, which caused sludge bulking, was inhibited. Paracoccus, a strong PHA producer, has been enriched to ensure that the maximum PHA synthesis of the system.

Simultaneous recovery of vivianite and produce short-chain fatty acids from waste activated sludge using potassium ferrate as pre-oxidation treatment.

Recovery resources from waste active sludge (WAS) is an effective way to alleviate the predicament of WAS disposal, and it is also conducive to the carbon neutralization of wastewater treatment systems. This study discussed the strategy of WAS anaerobic fermentation after pre-oxidation with potassium ferrate (K2FeO4, PF), which can simultaneously recover vivianite and enhance SCFAs production. The results showed that PF pre-oxidation considerably shortened the fermentation time of SCFAs to 2 days, and the main Fe-P mineral was vivianite. The optimal PF dosage of 0.06 g Fe (VI)/g TSS for pre-oxidation WAS resulted in the maximum SCFAs production and vivianite recovery rate of 3698.2 ± 118.98 mg COD/g VSS and 32.39%, respectively. The mechanism analysis showed that the oxidizing properties of PF significantly accelerated the disintegration of tight EPS, release of protein and sludge acidification efficiency. Moreover, the PF strengthened the transfer of P to the solid phase, forming the Fe-P mineral and unsaturated coordination state of phosphate group. Then the key microorganism Geobacter reduced the Fe3+ in Fe-P state to Fe2+ and combined unsaturated phosphate to form vivianite. This study provides an alternative method for resource recovery and environmentally friendly disposal of WAS and contributes to the carbon neutrality of urban water systems.

Evaluation of Fe(VI)/Fe(II) combined with sludge adsorbents in secondary effluent organic matter removal.

Wastewater reclamation and reuse are important methods that help to achieve an equilibrium within demand and offer, and also one of the important ways to reduce carbon emission. The existence of secondary effluent organic matter (EfOM) will bring potential threat to the environment in reuse process. Therefore, it is important to develop reclaimed water reuse technology that effectively remove EfOM. In this study, the removal of EfOM performance of ferrates enhanced by FeCl2 (Fe(VI)/Fe(II)) combined with sludge adsorbents (SAs) was evaluated by using the continuous-flow process (FeSDF), which was composed of Fe(VI)/Fe(II), SAs, densadeg and filtration. The results showed that when the inflow rate was 1 L/h, the optimal operating conditions of FeSDF including 5 mg/L of Fe(VI), 1 mg/L of Fe(II), 1 g/L of SA and 50% of the reflux ratio. Bulk organic indicators, including chemical oxygen demand, dissolved organic carbon, ammonia, total nitrogen, total phosphorus, turbidity, and ultraviolet absorbance at 254 nm in the effluent met the water quality standard for scenic environment use (GB/T 18921-2019 in China). The addition of Fe(II) makes the coagulation process by Fe(VI) produce more Fe(III) and produce more quality of sedimentary flocs and improve the removal efficiency of EfOM. The removal of organic micro-pollutants (OMPs) was mainly due to ferrate oxidation and SA adsorption in FeSDF, and the removal of most of the OMPs was more than 90%. The total fluorescence intensity removal efficiency in FeSDF was 63.8%. Moreover, the genotoxicity of the FeSDF effluent decreased to 0.73 µg 4-nitroquiniline-N-oxide/L, and the reduction efficiency reached 97.6%. The actual efficiency of most of the indicators is greater than the expected efficiency, indicating that there is a synergistic comprehensive effect during the whole process operation of FeSDF.

Response of antibiotic resistance to the co-exposure of sulfamethoxazole and copper during swine manure composting.

Heavy metals driven co-selection of antibiotic resistance in soil and water bodies has been widely concerned, but the response of antibiotic resistance to co-existence of antibiotics and heavy metals in composting system is still unknown. Commonly used sulfamethoxazole and copper were individually and jointly added into four reactors to explore their effects on antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), heavy metal resistance genes (MRGs) and bacterial community structure. The abundance of total ARGs and MGEs were notably decreased by 68.64%-84.95% and 91.27-97.38%, respectively, after the composting. Individual addition of sulfamethoxazole, individual addition of copper, simultaneously addition of sulfamethoxazole and copper increased the abundance of ARGs and MGEs throughout the composting period. Co-exposure of sulfamethoxazole and copper elevated the total abundance of ARGs by 1.17-1.51 times by the end of the composting compared to individual addition of sulfamethoxazole or copper. Network analysis indicated that the shifts in potential host bacteria determined the ARGs variation. Additionally, MGEs and MRGs had significant effects on ARGs, revealing that horizontal gene transfer and heavy metals induced co-resistance could promote ARGs dissemination.

Microbial community competition rather than high-temperature predominates ARGs elimination in swine manure composting.

Aerobic composting is commonly used in pig manure treatment, however, antibiotic resistance genes (ARGs) and their unclear transformation during composting process make the treated manure land using risky. The effects of enhanced thermophilic phase strategy (external heating (HTC) and thermophiles inoculation (MC)) on ARGs removal and the underlying mechanisms were investigated during swine manure composting. HTC increased the total relative abundance (RA) of ARGs by 32.38%, and MC decreased by 21.50% compared to CK by the end of the composting. Mantel test indicated that it was not temperature (P > 0.05), but environmental parameters (pH, Electric Conductivity (EC), etc.) and metabolic products (nitrogen forms) significantly affected the ARGs profile. Partial least-squares path modeling (PLS-PM) suggested that microbial community structure (bacterial abundance and diversities) was the main factor for ARGs evolution. Co-occurrence analysis revealed that HTC could promote the propagation of ARG hosts in later stage of the composting because the strong selection of thermophiles resulted in ecological niches vacancy, and MC enhanced the competition between hosts and nonhosts for ecological niches by increasing thermophiles diversities. These results suggested that competitive inhibition to potential ARGs hosts could be a helpful strategy in ARGs threaten elimination during composting.

Effects of H3PO4 modified biochar on heavy metal mobility and resistance genes removal during swine manure composting.

In this research, static composting treatments of swine manure with forced ventilation were conducted to study the effects of biochar (BC) and H3PO4 modified biochar (BP) addition on heavy metals (HMs) stabilization, profiles of antibiotic resistance genes (ARGs), heavy metals resistance genes (MRGs) and bacterial communities during swine manure composting. After 42 days of the composting, compared to control (CK), BC and BP decreased the concentration of diethylenetriamine pentaacetic acid extractable Cu and Zn by 12.04%, 15.15% and 26.91%, 36.50%, respectively. Furthermore, BC and BP treatments reduced the total abundances of nine ARGs by 4.02% and 66.21%, and five MRGs by 53.66% and 58.81%, compared to CK in the compost product. Network analysis and square structural equation model analysis revealed that the decrease of ARGs and MRGs in BP treatment was related tothe change in bacterial community during the composting, rather than differences in co-selection pressure.

Effect of KH2PO4-modified biochar on immobilization of Cr, Cu, Pb, Zn and as during anaerobic digestion of swine manure.

In this study, the immobilization performance and mechanisms of heavy metals (HMs) in swine manure (SM) during anaerobic digestion (AD) with biochar (BC) and KH2PO4-modified biochar (BP) were investigated. BC and BP addition decreased DTPA-extractable Cr, Cu, Pb and Zn amount, transformed these HMs to more stable state, and decreased the ecological risks of these HMs by 2 grades accordingly. BP exhibited a higher passivation efficiency for Cr, Cu and Pb and 5% -10% biochar dosage showed the maximum passivation effects. Characterization results showed that Cr, Cu and Pb immobilization with BP were mainly attributed to the formation of phosphate precipitation. However, both DTPA extraction and mobility of As increased with biochar addition, because the release of phosphorus in biochar had negative effect on As immobilization. BP could serve as a novel remediation agent for Cr, Cu, Pb and Zn passivation but special attention should be paid with As presence.

Effects of polyurethane foam carrier addition on anoxic/aerobic membrane bioreactor (A/O-MBR) for coal gasification wastewater (CGW) treatment: Performance and microbial community structure.

Coal gasification wastewater (CGW) is a typical toxic and refractory industrial wastewater with abundant phenols contained. Two identical anoxic/aerobic membrane bioreactors (with (R2) and without (R1) polyurethane (PU) foam) were carried out in parallel to investigate the role of PU foam addition in enhancing pollutants removal in CGW. Results showed that both systems exhibited effective removal of chemical oxygen demand (>93%) and total phenols (>97%) but poor ammonia nitrogen removal (<35%) constrained by ammonia oxidation process. GC-MS analysis revealed that aromatic and other refractory intermediates were dramatically reduced in R2. Moreover, the PU addition had negligible influence on the total soluble microbial products and extracellular polymeric substances contents but significantly alleviated membrane fouling with the operating time 33% prolonged. Microbial community revealed that Flavobacterium, Holophaga, and Geobacter were enriched on PU. Influent type might be a main driver for microbial community succession.

Effect of inoculum and organic loading on mixed culture polyhydroxyalkanoate production using crude glycerol as the substrate.

Two different sources of activated sludge were inoculated to select and enrich polyhydroxyalkanoate (PHA) producing culture from crude glycerol. The results showed that the sludge taken from the wastewater treatment plant with higher microbial diversity could enrich PHA producing culture with higher PHA synthesis capacity (25.93%) and specific PHA storage rate (0.27 mg COD/(mg·h)) in a short enrichment time, comparing to the sludge taken from the enriched PHA-producing culture using VFAs as the substrate. The enrichment performance under different organic loadings were investigated and similar microbial community composition, good operating stability and high PHA accumulation (SBR#1, 36.59%; SBR#2, 36.33%) was observed at 2000 mg COD/(L·d) when crude glycerol was used as the substrate. The maximum content of PHA was affected by the concentration of glycerol. Gardnerella was for the first time found to be the dominant genus in the PHA production system using crude glycerol. The research would guide the application of using crude glycerol resources for PHA production.